. . . GP (1000x) concentrate - 10 litre Drum

AlgaBoost Drum* AlgaBoost (+1000x) GP base is an 0.2 micron-filtered, 2 part, concentrate of GP medium base, for culture of marine microalgae.

* Makes: 10,000 litres of GP medium at final concentration.

* Nutrient Balance: When added to your seawater + freshwater mix (seawater : freshwater = 3 : 1 ) according to the accompanying instructions, this formulation will provide the following nutrient levels (Loeblich and Smith, 1968):

              
NutrientFinal conc per litre (g/l)
NO3-123 mg
K+93 mg
PO415.8 mg
BO3+32.5 mg
Fe3+0.3 mg
Mn2+1.2 mg
Co2+0.032 mg
Zn2+0.086mg
SeO32-0.0013 mg
Na+3.7 mg
Cl-12.7 mg
SO42-0.126 mg
Thiamine HCl0.1 mg
Vit B120.001 mg
Biotin0.002 mg
NutrientFinal conc per litre (g/l)
FeCl3.6H2O1.45 mg
KNO3200 mg
NaH2PO4.2H2034.8 mg
H3BO334.2 mg
EDTA.2H2030 mg
MnCl2.4H204.3 mg
CoCl2.6H200.13 mg
ZnSO4.7H200.364 mg
Biotin0.002 mg
Vit B120.001 mg
Thiamine HCl0.1 mg

* To Make GP base: Pour 1 litre from the 10 litre Drum (Part A) into a sterile culture bag. Add 10ml from the 100 ml bottle of Part B. Add 750 litres of sterile seawater + 250 litres of sterile fresh-water. Mix and aerate and you've got 1000 litres of GSe base

* To Make Full GP (with soil extract): Pour 1 litre from the 10 litre drum (Part A) into a sterile culture bag. Add 10ml from the 100 ml syringe of Part B. Add 5 litres of soil extract. Add 750 litres of sterile seawater + 250 litres of sterile fresh-water. Mix and aerate and you've got 1000 litres of Full GSe medium

* Reference: Loeblich, A.R and Smith, V.E. "Chloroplast pigments of the marine dinoflagellate Gyrodinium resplendens": Lipids 3: 5-13, 1968.

* Sterility preparation; concentrate is 0.2 micron filtered.100 ml + 100 litre seawater makes 100 litres silicate-free Harrisons medium    
* AlgaBoost is a trademark of AusAqua Pty Ltd
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