* 'AlgaBoost EPA-1002/1003 no EDTA' is a 400x concentrate of the medium used for Toxicity Testing. It is identical to AlgaBoost EPA-1002/1003, except for the absence of EDTA.

* Application : medium of choice for heavy metal toxicity tests; the exclusion of EDTA from this formulation allows more sensitive detection of heavy metals.

* When diluted in water according to instructions, it makes the following media:

        1. EPA Test Method 1002.0 (but EDTA free) (Daphnid Ceriodaphnia dubia, Survival and Reproduction Test).

        2. EPA Test Method 1003.0 (but EDTA free) (Green Alga, Selenastrum capricornutum, Growth Test).

* How is the product supplied ?   - As a 2-part mix, in packs containing 12 kits (enough to make 12 x 2 litres of EPA 1002/1003 medium).  Each part of the 2-part mix is sterile and is provided in a convenient 5ml syringe; the two syringes are provided in a sealed sterile pack.

* To Make the Medium:    - Open a 2-syringe kit.  Then add the the contents of one 5ml syringe to 2 litres of pure water, mix thoroughly, then add contents of the other 5 ml syringe and mix (instructions accompany the product).  Aerate the mixture, adjust pH if necessary (recommended for EPA method 1003.0) and filter sterilise. 

NOTE: EPA-1002/1003 is sterile; the final sterilisation step (described above) is necessary only if diluent (water) is not sterile and/or if sterile technique has not been used while preparing the final medium.

* Reference (EDTA-version): "Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms." Eds: P.A. Lewis, D.J. Klenni, J.M Lazorchak, T.J. Norberg-King, W.H. Peltier, M.A. Heber 1994.